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This work was supported by grants CA Y. Conflict of interest: The authors have declared that no conflict of interest exists. National Center for Biotechnology Information , U. Bio Protoc. Author manuscript; available in PMC Jun Todd V.
Author information Copyright and License information Disclaimer. Copyright notice. The publisher's final edited version of this article is available at Bio Protoc. See other articles in PMC that cite the published article. Abstract Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. Background Paramount to tracking cells in vivo is the ability to detect them externally by minimally invasive methods.
Open in a separate window. Figure 1.
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Note: The same 10 ml of gelatin solution can be reused to coat several plates. Note: We prepare 4 plates for each lentivirus preparation. Notes: Lentiviruses are classified as Biosafety Level 2 BSL-2 organisms due to their ability to infect primary human cells and experiments need to be conducted in appropriate facilities. Remove 2 ml of media from each plate now 8 ml total. Concentrate the virus.
Note: The pellet will be small and can be difficult to visualize. Discard flow through. Figure 2. Figure 3. Confirmation of luciferase expression A.
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Figure 4. Data analysis IVIS data is collected in regions of interest and can be exported to Excel spreadsheet for analysis. Acknowledgments This work was supported by grants CA Y. Footnotes Conflict of interest: The authors have declared that no conflict of interest exists. J Clin Invest. Analyses of tumor burden in vivo and metastasis ex vivo using luciferase-expressing cancer cells in an orthotopic mouse model of neuroblastoma.
Methods Mol Biol. Natural expression of the CD19 antigen impacts the long-term engraftment but not antitumor activity of CDspecific engineered T cells.
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J Immunol. Adoptive immunotherapy of experimental autoimmune encephalomyelitis via T cell delivery of the IL p40 subunit. Firefly luminescence: a historical perspective and recent developments. Photochem Photobiol Sci. Noninvasive imaging of reporter gene expression in living subjects. Adv Cancer Res. De novo methylation and expression of retroviral genomes during mouse embryogenesis. Bioluminescent human breast cancer cell lines that permit rapid and sensitive in vivo detection of mammary tumors and multiple metastases in immune deficient mice.
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